Queen's University Environmental DNA Workshop 2025

Stylized Horizontal Image of DNA

Where: Queen's University Biological Station - Opinicon Campus

When: Arriving Sunday, May 11th to Friday, May 16th 2025. An additional 1-day Oxford Nanopore workshop can be added in registration

Target audience: professional biologists in governmental or consulting sectors, NGO personnel, senior undergraduates and grad students seeking additional skills

Registration: Meals & accommodations and all course materials included.

Students $950, General Public/Professionals $1,250

Transportation to/from Kingston and the Biological Station is included (from Queen's University main campus or Kingston bus or train stations)

Participants will receive a Queen's University micro-credential.

REGISTER HERE

Synopsis: Environmental DNA (eDNA) is becoming a mainstay of non-invasive, reliable, and cost-effective surveys of at-risk and invasive species, pathogen detection, and biodiversity assessment. These approaches rely on the fact that organisms leave their DNA in the environment, making it possible to detect their presence without capture or even direct observation. Reliable eDNA data depend on field and lab rigorous technique because significant biases can occur through the process from the sampling to the data processing, and they greatly influence species detection results.

Drone shot of water sampling
Water sampling on the St. Lawrence. Photo. Allen Tian

This eDNA workshop will provide a broad overview of the approaches used in eDNA studies for both single-species and multi-species detections. This five-day course will benefit those interested in undertaking or overseeing eDNA studies, or evaluating research proposals and research, especially in management and monitoring applications. The workshop includes lectures, field and lab demonstrations, and hands-on training in analysis and interpretation of data, but does not require detailed knowledge of genetics or molecular biology. We offer a background on sources and fates of eDNA  in the environment, methods and applications with emphasis on design, implementation, analysis and interpretation of species-specific and multi-species eDNA approaches. We will cover advantages and limitations of each, as well as the precautions needed through the whole process to get robust data. We will provide a manual that includes method overviews, field and lab protocols, links to key web resources, and useful literature. Some of the case studies we will present are from our own lab and particularly those supported by an NSERC Alliance grant where we are working with multiple partners (e.g. River Institute, Thousand Islands National Park, Mohawk Council of Akwesasne Environment Program, First Nations Technical Institute, Invasive Species Council).

Instructors (click on names for bios): Dr. Dilini Abeyrama, Dr. Bojian ChenAllen Tian

Consultants: Dr. Steve LougheedDr. Yuxiang Wang, Dr. Orianne Tournayre, Stafford Maracle

Guest speakers: Dr. Bob Hanner (University of Guelph) and Dr. Melania Cristescu (McGill University)


Timetable

DATETIMEACTIVITYPRESENTER
SUN, May 11Late afternoonArrival at QUBS. Settling into accommodationsNA
 5:30-6:30 PMDinner 
 7:00-8:00 PMTalk: Welcome to QUBS - Introductions, Introduction to eDNAStephen Lougheed
MON, May 127:30-8:30 AMBreakfast 
 9:00-10:15 AMTalk: Introduction to eDNA study designDilini Abeyrama
 10:15-10:30 AMBreak 
 10:30 AM-noonTalk: Overview of eDNA-focused fieldworkDilini
 12:00-1:00 PMLunch 
 1:00-2:00 PMTalk: Introduction to qPCR, ddPCRDilini
 2:00-2:30 PMBreak and preparation for field activities 
 2:30-5:30 PMField practicum: Sampling water and frog swabs + metadata collection + water filtrationAllen Tian
 5:30-6:30 PMDinner 
 7:00-9:00 PMDNA extraction part 1 (Talk & Lab  Practicum) - QIAGEN KitAllen
TUES, May 137:30-8:30 AMBreakfast 
 9:00-11:00 AMLab Practicum: DNA Extraction. Part 2Allen
 11:00 AM-noonDemo: Bio-RAD qPCR Demonstration Part 1Andre & Amir from BioRad
 12:00-1:00 PMLunch 
 1:00-3:15 PMDemo: Bio-RAD qPCR Demonstration Part 2Andre & Amir
 3:15-3:45 PMBreak 
 3:45-5:00 PMDemo: Bio-RAD qPCR Demonstration Part 3Andre & Amir
 5:30-6:30 PMDinner 
 7:00-8:00 PMTalk: Data analysis & single species detection recap, Q&AAllen
WED, May 147:30-8:30 AMBreakfast 
 8:30-10:00 AMTalk: Broad overview of eDNA metabarcodingBojian Chen
 10:00-10:30 AMBreak 
 10:30 AM - noon Practicum: Polymerase chain reaction with MiFish primersAllen & Dilini
 12:00-1:00 PMLunch 
 1:00-2:30 PMTalk: Library Prep and metabarcoding workflow specificsBojian
 2:30-3:00 PMBreak 
 3:00-5:00 PMTalk: DNA visualization: gels, gel excision, Nanodrop/QubitAllen & Dilini
 5:30-6:30 PMDinner 
 7:00-8:00 PMGuest seminar: Dr. Melania Cristescu 
THURS, May 157:30-8:30 AMBreakfast 
 8:30-10:00 AMTalk: Introduction to eDNA metabarcoding bioinformaticsBojian
 10:00-10:15 AMBreak 
 10:15 AM - noonPracticum: Command line & getting into BASHAllen
 12:00-1:00 PMLunch 
 1:00- 2:30 PMPracticum: Bioinformatics analysis & interpretation of metabarcoding results. Part 1Allen
 2:30-3:00 PMBreak 
 3:00-4:30 PMPracticum: Bioinformatics analysis & interpretation of metabarcoding results. Part 2Allen
 4:30-5:30 PMGuest seminar: Dr. Bob Hanner 
 5:30-6:30 PMDinner 
 7:00-8:00 PMQ&A: eDNA standards & roundtable on quality control 
FRI, May 167:30-8:30 AMBreakfast 
 8:30-10:00 AMFinal quiz & wrap-up