Where: Queen's University Biological Station - Opinicon Campus
When: Arriving Sunday, May 12th to Friday, May 17th 2024
Target audience: professional biologists in governmental or consulting sectors, NGO personnel, senior undergraduates and grad students seeking additional skills
Registration: Meals & accommodations included.
Students $875, General Public/Professionals $1150
Transportation to/from Kingston and the Biological Station is included (from Queen's University main campus or Kingston bus or train stations)
Participants will receive a Queen's University micro-credential.
Synopsis: Environmental DNA (eDNA) is becoming a mainstay of non-invasive, reliable, and cost-effective surveys of at-risk and invasive species, pathogen detection, and biodiversity assessment. These approaches rely on the fact that organisms leave their DNA in the environment, making it possible to detect their presence without capture or even direct observation. Reliable eDNA data depend on field and lab rigorous technique because significant biases can occur through the process from the sampling to the data processing, and they greatly influence species detection results.

This eDNA workshop will provide a broad overview of the approaches used in eDNA studies for both single-species and multi-species detections. This five-day course will benefit those interested in undertaking or overseeing eDNA studies, or evaluating research proposals and research, especially in management and monitoring applications. The workshop includes lectures, field and lab demonstrations, and hands-on training in analysis and interpretation of data, but does not require detailed knowledge of genetics or molecular biology. We offer a background on sources and fates of eDNA in the environment, methods and applications with emphasis on design, implementation, analysis and interpretation of species-specific and multi-species eDNA approaches. We will cover advantages and limitations of each , as well as the precautions needed through the whole process to get robust data. We will provide a manual that includes method overviews, field and lab protocols, links to key web resources, and useful literature.
Instructors (click on names for bios): Lectures. Dr. Dilini Abeyrama, Dr. Bojian Chen; Labs. Allen Tian, Stafford Maracle
Consultants: Dr. Steve Lougheed, Dr. Yuxiang Wang, Dr. Orianne Tournayre
Guest speakers: Dr. Bob Hanner (University of Guelph) and Dr. Cath Abbott (Fisheries and Oceans Canada)
DAY | TIMES | ACTIVITY |
Sunday May 12 | Late afternoon | Arrival at QUBS - meet at the Raleigh J. Robertson Biodiversity Centre |
5:30-6:30 PM | Dinner | |
7:00-8:00 PM | Welcome to QUBS and Introductions | |
Monday May 13 | 7:30-8:30 AM | Breakfast |
9:00-10:15 AM | Presentation: Introduction to eDNA study design | |
10:15-10:30 AM | Break | |
10:30AM-noon | Presentation: Overview of fieldwork, sampling, filtration, transport, storage & extraction | |
12:00-1:00 PM | Lunch | |
1:00-2:15 PM | Presentation: Introduction to assays: quantitative PCR, digital PCR & metabarcoding | |
2:15-2:30 PM | Break | |
2:30-5:30 PM | Field practicum: Sampling water + metadata collection + water filtration | |
5:30-6:30 PM | Dinner | |
7:00-8:30 PM | Lab demo: DNA extraction. | |
Tuesday May 14 | 7:30-8:30 AM | Breakfast |
9:00-11:00 AM | Lab Practicum: DNA extraction of samples collected Monday - (QIAGEN Powerwater kit, QIAGEN Powersoil kit) part 1 | |
11:00-11:15 AM | Break | |
11:15-12:15 | BioRad Demo: qPCR & ddPCR Part 1 | |
12:00-1:00 PM | Lunch | |
1:00-3:30 PM | BioRad Demo: qPCR & ddPCR Part 2 | |
3:15-3:30 PM | Break | |
3:30-5:30 PM | BioRad Demo: qPCR & ddPCR Part3 | |
5:30-6:30 PM | Dinner | |
7:00-8:00 PM | Presentation: Data analysis and interpretation of qPCR results, with a case study. | |
Wednesday May 15 | 7:30-8:30 AM | Breakfast |
9:00-10:30 AM | Presentation: Overview of metabarcoding + metabarcoding step-by-step library preparation (Part 1) | |
10:30-10:45 AM | Break | |
10:45-noon | Lab practicum: Library preparation (Part 1) | |
noon-1 PM | Lunch | |
1-2:15 PM | Presentation : Metabarcoding step-by-step library preparation (Part 2) | |
2:15-2:30 PM | Break | |
2:30-5:00 PM | Lab practicum: Library preparation & gel purification (Part 2) | |
5:30-6:30 PM | Dinner | |
7:00-8:00 PM | QUBS weekly seminar. Dr. Bob Hanner. | |
Thursday May 16 | 7:30-8:30 AM | Breakfast |
8:30 - 9:30 AM | Presentation: Introduction to eDNA metabarcoding bioinformatics | |
9:30-10:00 AM | Break | |
10:00-11:00 AM | Presentation: Basic command line | |
11:00-noon | Practicum: Basic command line | |
noon-1 PM | Lunch | |
1:00-3:00 PM | Practicum: Bioinformatics. Part 1 | |
3:00-3:30 PM | Break | |
3:30-4:30 PM | Practicum: Bioinformatics - analysis & interpretation of metabarcoding results. Part 2 | |
4:30-5:30 PM | Guest lecture. Dr. Cath Abbott. The twisty-turny road towards translating eDNA science into action | |
5:30-6:30 PM | Dinner | |
6:30-7:30 PM | Q & A with Dr. Abbott | |
7:30-8:00 PM | Quiz | |
Friday May 17 | 7:30-8:30 AM | Breakfast |
9:00 AM-noon | Quiz results & debrief. Depart |