Queen's University Environmental DNA Workshop 2024

eDNA Workshop 2024

Where: Queen's University Biological Station - Opinicon Campus

When: Arriving Sunday, May 12th to Friday, May 17th 2024

Target audience: professional biologists in governmental or consulting sectors, NGO personnel, senior undergraduates and grad students seeking additional skills

Registration: Meals & accommodations included.

Students $875, General Public/Professionals $1150

Transportation to/from Kingston and the Biological Station is included (from Queen's University main campus or Kingston bus or train stations)

Participants will receive a Queen's University micro-credential.

REGISTER HERE

Synopsis: Environmental DNA (eDNA) is becoming a mainstay of non-invasive, reliable, and cost-effective surveys of at-risk and invasive species, pathogen detection, and biodiversity assessment. These approaches rely on the fact that organisms leave their DNA in the environment, making it possible to detect their presence without capture or even direct observation. Reliable eDNA data depend on field and lab rigorous technique because significant biases can occur through the process from the sampling to the data processing, and they greatly influence species detection results.

Drone shot of water sampling
Water sampling on the St. Lawrence. Photo. Allen Tian

This eDNA workshop will provide a broad overview of the approaches used in eDNA studies for both single-species and multi-species detections. This five-day course will benefit those interested in undertaking or overseeing eDNA studies, or evaluating research proposals and research, especially in management and monitoring applications. The workshop includes lectures, field and lab demonstrations, and hands-on training in analysis and interpretation of data, but does not require detailed knowledge of genetics or molecular biology. We offer a background on sources and fates of eDNA  in the environment, methods and applications with emphasis on design, implementation, analysis and interpretation of species-specific and multi-species eDNA approaches. We will cover advantages and limitations of each , as well as the precautions needed through the whole process to get robust data. We will provide a manual that includes method overviews, field and lab protocols, links to key web resources, and useful literature.

Instructors (click on names for bios): Lectures. Dr. Dilini Abeyrama, Dr. Bojian Chen; Labs. Allen Tian, Stafford Maracle

Consultants: Dr. Steve LougheedDr. Yuxiang Wang, Dr. Orianne Tournayre

Guest speakers: Dr. Bob Hanner (University of Guelph) and Dr. Cath Abbott (Fisheries and Oceans Canada)

Workshop Timetable
DAYTIMESACTIVITY
Sunday May 12Late afternoonArrival at QUBS - meet at the Raleigh J. Robertson Biodiversity Centre
 5:30-6:30 PMDinner
 7:00-8:00 PMWelcome to QUBS and Introductions
Monday May 137:30-8:30 AMBreakfast
 9:00-10:15 AMPresentation: Introduction to eDNA study design
 10:15-10:30 AMBreak
 10:30AM-noonPresentation: Overview of fieldwork, sampling, filtration, transport, storage & extraction
 12:00-1:00 PMLunch
 1:00-2:15 PMPresentation: Introduction to assays: quantitative PCR, digital PCR & metabarcoding
 2:15-2:30 PMBreak
 2:30-5:30 PMField practicum: Sampling water + metadata collection + water filtration
 5:30-6:30 PMDinner
 7:00-8:30 PMLab demo: DNA extraction.
Tuesday May 147:30-8:30 AMBreakfast
 9:00-11:00 AMLab Practicum: DNA extraction of samples collected Monday - (QIAGEN Powerwater kit, QIAGEN Powersoil kit) part 1
 11:00-11:15 AMBreak
 11:15-12:15BioRad Demo: qPCR & ddPCR Part 1
 12:00-1:00 PMLunch
 1:00-3:30 PMBioRad Demo: qPCR & ddPCR Part 2
 3:15-3:30 PMBreak
 3:30-5:30 PMBioRad Demo: qPCR & ddPCR Part3
 5:30-6:30 PMDinner
 7:00-8:00 PMPresentation: Data analysis and interpretation of qPCR results, with a case study.
Wednesday May 157:30-8:30 AMBreakfast
 9:00-10:30 AMPresentation: Overview of metabarcoding + metabarcoding step-by-step library preparation (Part 1)
 10:30-10:45 AMBreak
 10:45-noonLab practicum: Library preparation (Part 1)
 noon-1 PMLunch
 1-2:15 PMPresentation : Metabarcoding step-by-step library preparation (Part 2)
 2:15-2:30 PMBreak
 2:30-5:00 PMLab practicum: Library preparation & gel purification (Part 2)
 5:30-6:30 PMDinner
 7:00-8:00 PMQUBS weekly seminar. Dr. Bob Hanner.
Thursday May 167:30-8:30 AMBreakfast
 8:30 - 9:30 AMPresentation: Introduction to eDNA metabarcoding bioinformatics
 9:30-10:00 AMBreak
 10:00-11:00 AMPresentation: Basic command line
 11:00-noonPracticum: Basic command line
 noon-1 PMLunch
 1:00-3:00 PMPracticum: Bioinformatics. Part 1
 3:00-3:30 PMBreak
 3:30-4:30 PMPracticum: Bioinformatics - analysis & interpretation of metabarcoding results. Part 2
 4:30-5:30 PMGuest lecture. Dr. Cath Abbott. The twisty-turny road towards translating eDNA science into action
 5:30-6:30 PMDinner
 6:30-7:30 PMQ & A with Dr. Abbott
 7:30-8:00 PM Quiz
Friday May 177:30-8:30 AM Breakfast
 9:00 AM-noon Quiz results & debrief. Depart