eDNA Workshop Timetable

 

Meals & Accommodations at the Queen's University Biological Station for participants is included in the registration fee.

 
DAY TIMES ACTIVITY
Sunday Late afternoon Arrival at QUBS
  5:30-6:30 PM Dinner
  7:00-8:00 PM Welcome to QUBS - Introductions
Monday 7:30-8:30 AM Breakfast
  9:00-10:15 AM Presentation: Overview of eDNA (Lougheed)
  10:15-10:30 AM Break
  10:30AM-noon Presentation: Introduction to eDNA study design, qPCR, ddPCR (Tian)
  12:00-1:00 PM Lunch
  1:00-2:15 PM Presentation: Overview of fieldwork, sampling, filtration, transport, storage, extraction (Tian)
  2:15-2:30 PM Break
  2:30-5:30 PM Field practicum: Sampling water, sediment, and soil + metadata collection + water filtration (Tian & Maracle)
  5:30-6:30 PM Dinner
  7:00-8:00 PM Guest Speaker: Dr. Robert Hanner (Guelph University). 
Tuesday 7:30-8:30 AM Breakfast
  9:00-10:15AM Practicum: DNA extraction of samples collected the day before - (QIAGEN Powerwater kit, QIAGEN Powersoil kit) part 1
  10:15-10:30AM Break
  10:30-12:00 Practicum: DNA extraction of samples collected the day before - (QIAGEN Powerwater kit, QIAGEN Powersoil kit) part 2
  12:00-1:00PM Lunch
  1:00-3:15PM Practicum: Bio-RAD qPCR demo: qPCR. While the qPCR runs, presentation on capabilities of qPCR and dPCR.
  3:15-3:30PM Break
  3:30-5:30PM Lecture: Bio-RAD. Interpretation of qPCR results. Second part of session. Primer design for qPCR (and for ddPCR).
  5:30-6:30PM Dinner
  7:00-8:00 PM Lecture: Data analysis and interpretation of qPCR results, with a case study (Tian)
Wednesday 7:30-8:00 AM Breakfast
  8:00 - 9:40 Lecture: Overview of metabarcoding and high throughput sequencing platforms. (Maracle)
  9:40-9:45 AM Break
  9:45-noon Lab practicum: Polymerase chain reaction: Prepare mix and run PCR (Tian & Maracle)
  noon-1PM Lunch
  1-3:30 PM Lecture: Presentation: Library prep and DNA quantification (Tian)
  3:30-4:00 PM Break
  4:00-5:00 PM Practicum: Loading & Running agarose gels. Estimating insert size. Qubit + Nanodrop.
  5:30-6:30 PM Dinner
  7:00-8:00 PM QUBS weekly seminar. Dr. Chris Wilson
Thursday 7:30-8:30 AM Breakfast
  8:30 - 9:30 AM Lecture: General introduction to command line interface
  9:30 -10:00 AM Break
  10:00 - 11:30 Practicum: Practicum: Command line troubleshooting (Tian & Maracle)
  11:30- 12:30 Lecture: Bioinformatics data interpretation
  12:30 -1:30 PM Lunch
  1:30-3:00 PM Practicum: Bioinformatics analysis & interpretation of metabarcoding results. Part 1 (Maracle)
  3:00-3:30 PM Break
  3:30-5:30 PM Practicum: Bioinformatics analysis & interpretation of metabarcoding results. Part 2 (Maracle)
  5:30-6:30 PM Dinner
  7:00-8:30 PM Quiz
Friday 7:30-8:30 AM Breakfast
  9:00 AM-noon  Quiz results and debrief. Depart