Meals & Accommodations at the Queen's University Biological Station for participants is included in the registration fee.
| DAY | TIMES | ACTIVITY |
| Sunday | Late afternoon | Arrival at QUBS |
| 5:30-6:30 PM | Dinner | |
| 7:00-8:00 PM | Welcome to QUBS - Introductions | |
| Monday | 7:30-8:30 AM | Breakfast |
| 9:00-10:15 AM | Presentation: Overview of eDNA (Lougheed) | |
| 10:15-10:30 AM | Break | |
| 10:30AM-noon | Presentation: Introduction to eDNA study design, qPCR, ddPCR (Tian) | |
| 12:00-1:00 PM | Lunch | |
| 1:00-2:15 PM | Presentation: Overview of fieldwork, sampling, filtration, transport, storage, extraction (Tian) | |
| 2:15-2:30 PM | Break | |
| 2:30-5:30 PM | Field practicum: Sampling water, sediment, and soil + metadata collection + water filtration (Tian & Maracle) | |
| 5:30-6:30 PM | Dinner | |
| 7:00-8:00 PM | Guest Speaker: Dr. Robert Hanner (Guelph University). | |
| Tuesday | 7:30-8:30 AM | Breakfast |
| 9:00-10:15AM | Practicum: DNA extraction of samples collected the day before - (QIAGEN Powerwater kit, QIAGEN Powersoil kit) part 1 | |
| 10:15-10:30AM | Break | |
| 10:30-12:00 | Practicum: DNA extraction of samples collected the day before - (QIAGEN Powerwater kit, QIAGEN Powersoil kit) part 2 | |
| 12:00-1:00PM | Lunch | |
| 1:00-3:15PM | Practicum: Bio-RAD qPCR demo: qPCR. While the qPCR runs, presentation on capabilities of qPCR and dPCR. | |
| 3:15-3:30PM | Break | |
| 3:30-5:30PM | Lecture: Bio-RAD. Interpretation of qPCR results. Second part of session. Primer design for qPCR (and for ddPCR). | |
| 5:30-6:30PM | Dinner | |
| 7:00-8:00 PM | Lecture: Data analysis and interpretation of qPCR results, with a case study (Tian) | |
| Wednesday | 7:30-8:00 AM | Breakfast |
| 8:00 - 9:40 | Lecture: Overview of metabarcoding and high throughput sequencing platforms. (Maracle) | |
| 9:40-9:45 AM | Break | |
| 9:45-noon | Lab practicum: Polymerase chain reaction: Prepare mix and run PCR (Tian & Maracle) | |
| noon-1PM | Lunch | |
| 1-3:30 PM | Lecture: Presentation: Library prep and DNA quantification (Tian) | |
| 3:30-4:00 PM | Break | |
| 4:00-5:00 PM | Practicum: Loading & Running agarose gels. Estimating insert size. Qubit + Nanodrop. | |
| 5:30-6:30 PM | Dinner | |
| 7:00-8:00 PM | QUBS weekly seminar. Dr. Chris Wilson | |
| Thursday | 7:30-8:30 AM | Breakfast |
| 8:30 - 9:30 AM | Lecture: General introduction to command line interface | |
| 9:30 -10:00 AM | Break | |
| 10:00 - 11:30 | Practicum: Practicum: Command line troubleshooting (Tian & Maracle) | |
| 11:30- 12:30 | Lecture: Bioinformatics data interpretation | |
| 12:30 -1:30 PM | Lunch | |
| 1:30-3:00 PM | Practicum: Bioinformatics analysis & interpretation of metabarcoding results. Part 1 (Maracle) | |
| 3:00-3:30 PM | Break | |
| 3:30-5:30 PM | Practicum: Bioinformatics analysis & interpretation of metabarcoding results. Part 2 (Maracle) | |
| 5:30-6:30 PM | Dinner | |
| 7:00-8:30 PM | Quiz | |
| Friday | 7:30-8:30 AM | Breakfast |
| 9:00 AM-noon | Quiz results and debrief. Depart |