eDNA Workshop Timetable

 

Meals & Accommodations at the Queen's University Biological Station for participants is included in the registration fee.

 
DAY TIMES ACTIVITY
Monday Late afternoon Arrival at QUBS
  5:30-6:30 PM Dinner
  7:00-8:00 PM Welcome to QUBS 
Tuesday 7:30-8:30 AM Breakfast
  9:00-10:15AM Presentation. Overview of eDNA 
  10:15-10:30AM Break
  10:30-12:00 Introduction to qPCR, ddPCR, metabarcoding
  12:00-1:00PM Lunch
  1:00-2:15PM Presentation: Sampling protocols, sample transport, and storage.+ water filtration methods 
  2:15-2:30PM Break
  2:30-5:30PM Field practicum: Sampling  water, sediment, and soil + metadata collection + water filtration . 
  5:30-6:30PM Dinner
  7:00-8:00 PM Guest research talk Dr. Elizabeth Clare (York University)
Wednesday 7:30-8:00 AM Breakfast
  8:00 - 9:40 DNA extraction of samples collected the day before -  (QIAGEN Powerwater kit, QIAGEN Powersoil kit)
  9:40-9:45 AM Break
  9:45-noon Bio-RAD ddPCR & qPCR demo : prepare qPCR & ddPCR mastermix, generate droplets and start the PCR reaction (qPCR and ddPCR).
  noon-1PM Lunch
  1-3:30 PM Bio-RAD  Lecture, PCR plate in the ddPCR reader and discuss/analyze the data (qPCR and ddPCR)
  3:30-4:00 PM Break
  4:00-5:00 PM Data analysis & interpretation qPCR & dPCR more in details = exercise (how to interpret results showing different case scenario?)
  5:30-6:30 PM Dinner
  7:00-8:00 PM QUBS weekly seminar.  Dr. Robert Hanner (Guelph University)
Thursday 7:30-8:30 AM Breakfast
  8:30 - 9:30 AM Lab practicum. Polymerase chain reaction: prepare mix and run PCR
  9:30 -10:00 AM Break
  10:00 - 11:30 While PCR is running, lecture on quantification methods (nanodrop, qubit) + start preparing gel
  11:30- 12:30  Loading & Running agarose gels, Estimating insert size. 
  12:30 -1:30 PM Lunch
  1:30-3:00 PM Bioinformatics analysis & interpretation of metabarcoding results. Part 1
  3:00-3:30 PM Break
  3:30-5:30 PM Bioinformatics analysis & interpretation of metabarcoding results. Part 2
  5:30-6:30 PM Dinner
  7:00-8:30 PM Debrief & summary. Exam. Workshop evaluation.
Friday 7:30-8:30 AM Breakfast
  9:00-10:00 AM  Exam results+ Depart