eDNA Workshop Timetable

This schedule is tentative as we firm up speakers and activities.

DAY TIMES ACTIVITY
Monday Late afternoon Arrival at QUBS
  5:30-6:30 PM Dinner
  7:00-8:00 PM Welcome to QUBS
Tuesday 7:30-8:30 AM Breakfast
  9:00-10:15 AM Presentation. Overview of eDNA (definition, sources, factors that shape its fate and duration). 
  10:15-10:45 AM Break
  10:45-noon Presentation: Sampling protocols, sample transport, and storage. 
  noon-1:00 PM Lunch
  1:00-3:30 PM Field practicum: Sampling protocols for water, sediment, and soil + metadata collection 
  3:30-4:00 PM  Break
  4:00-5:15 PM Presentation: Principles and applications of quantative PCR & metabarcoding
  5:30-6:30 PM Dinner
  7:00-8:00 PM Guest research talk on eDNA. TBA
Wednesday 7:30-8:30 AM Breakfast
  9:00-10:15 PM Lab practicum. Overview of DNA extraction & hands-on lab work
  10:15-10:45 AM Break
  10:45-noon Lab practicum. DNA extraction (QIAGEN, phenol-chloroform)
  noon-1:00 PM Lunch
  1:00-2:30 PM Lab practicum cont’d. DNA Extraction & quantification.
  2:30-3:00 PM Break
  3:00-5:00 PM TBA
  5:30-6:30 PM Dinner
  7:00-8:00 PM QUBS weekly seminar. TBA
Thursday 7:30-8:30 AM Breakfast
  9:00-10:15 AM Lab practicum. Polymerase chain reaction. Running agarose gels, Estimating insert size. 
  10:15-10:45 AM Break
  10:45-noon Data analysis & interpretation qPCR & dPCR.
  Noon-1:00 PM Lunch
  1:00-2:30 PM Bioinformatics analysis & interpretation of metabarcoding results. Part 1
  2:30-3:00 PM Break
  3:00-5:00 PM Bioinformatics analysis & interpretation of metabarcoding results. Part 2
  5:30-6:30 PM Dinner
  7:00-8:30 PM Debrief & summary. Presentation of certificates. Workshop evaluation.
Friday 7:30-8:30 AM Breakfast
  9:00-10:00 AM  Depart

 

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